In this probe, at first we examined the best route and dosage of
arginine administration on wound healing in an excisional
wound model in rats. Next, we intend to assess the impact of
photobiomodulation (
PBM) and
arginine, individually and together, on the wound healing. In the pilot study, an excisional
wound was made in each of 24 rats. There were 4 groups. Group 1 was the control group. In groups 2 and 3,
wounds were topically treated with
arginine ointments (ARG.) 2% and 5%, respectively. In group 4,
arginine was injected (ARG. INJ.,i.p.). In the main phase, in 24 new rats, an excisional
wound was made. There were 4 groups: group 5 served as the control.
Wounds in group 6 were topically treated with ARG 2%.
Wounds in group 7 were subjected to
PBM.
Wounds in group 8 were treated with PBM+ARG. 2%. On day 15,
wound area measurement,
wound strength, and stereological examination were performed. In the pilot study, we found that the ARG 2%
ointment significantly decreased
wound area than ARG. 5%, ARG. INJ. and control groups, and significantly increased
wound strength compared to the control and ARG.5% groups. In the main phase, a significant decrease of
wound area in all treatment regimens was induced. PBM + ARG. 2% and
PBM treatment regimens significantly improved
wound strength and almost all stereological parameters, compared to the control and ARG. 2% groups. PBM + ARG. 2% induced anti-inflammatory and angiogenic activities, and hastened the wound healing process in an excisional
wound model in rats.