Correlation of the serial-dilution protamine sulfate test with models of "hypercoagulability" and thrombosis in dogs.

Abstract

In a dog model, combinations of venous-injury thrombosis, homologous serum-induced "hypercoagulability," and fibrinolytic blockade were evaluated to determine the factors involved in the generation of a positive serial-dilution protamine sulfate test. Stasis and operative procedures did not produce a positive test. Small local thrombi induced by electrical current produced a positive test that tended to persist only during the initial formation and propagation of the thrombus, and was blocked by pretreatment with epsilon-aminocaproic acid, suggesting in this model that the test was detecting mainly early fibrin degradation products. Homologous serum infusion without venous-injury thrombosis also produced positive serial-dilution protamine sulfate test results that could not be blocked with epsilon-aminocaproic acid suggesting here that the serial-dilution protamine sulfate test was detecting mainly soluble complexes of fibrin monomer rather than early fibrin degradation products. The serial-dilution protamine sulfate test is therefore capable of detecting both small local thrombi and serum-induced "hypercoagulability" without thrombosis in the dog model.

• Authors: P B Francis, J E Wilson 3rd
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 67 Issue 4 Pg. 356-61 (Apr 1977) ISSN: 0002-9173 [Print] England
• PMID: 322471 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Protamines

Topics

• Animals
• Disease Models, Animal
• Dogs
• Indicator Dilution Techniques
• Protamines
• Thrombosis (diagnosis, etiology)