Circ_0001178 regulates miR-382/VEGFA axis to facilitate hepatocellular carcinoma progression.

Abstract

Circular RNAs (circRNAs) have been reported to regulate the gene expression through sponging corresponding microRNAs in multiple malignant tumors, including hepatocellular carcinoma (HCC). Up to now, the effects of circ_0001178 in HCC are barely known. In our current work, we tested circ_0001178 expression in HCC tissues and HCC cells and found it was greatly elevated. Then, we evaluated the function of circ_0001178 on HCC cell proliferation. We found HepG2 and Huh-7 cell proliferation was repressed after circ_0001178 shRNA was infected into the cells. Moreover, flow cytometry evidenced that HepG2 and Huh-7 cell apoptosis was markedly triggered and cell cycle was arrested. Meanwhile, it was shown that HCC cell migration and invasion capacity were markedly inhibited by loss of circ_0001178. Knockdown of circ_0001178 restrained HCC tumor growth in vivo. Then, miR-382 was predicted and confirmed as the target of circ_0001178. Circ_0001178 was demonstrated to modulate miR-382 expression negatively. The effect of circ_0001178 on HCC tumor was rescued by miR-382 overexpression. Furthermore, vascular epithelial growth factor A (VEGFA) is identified in various cancers. Currently, VEGFA was proved to be the downstream target of miR-382. To conclude, this research revealed that circ_0001178 induced HCC progression via modulating miR-382 and VEGFA axis.

• Authors: Shan Gao, Wei Hu, Xin Huang, Xingyue Huang, Wenwei Chen, Lidan Hao, Zubing Chen, Jian Wang, Hailiang Wei
• Journal: Cellular signalling (Cell Signal) Vol. 72 Pg. 109621 (08 2020) ISSN: 1873-3913 [Electronic] England
• PMID: 32240747 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020. Published by Elsevier Inc.

Chemical References

• MIRN382 microRNA, human
• MicroRNAs
• RNA, Circular
• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Apoptosis (genetics)
• Base Sequence
• Carcinoma, Hepatocellular (genetics, pathology)
• Cell Cycle (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Gene Expression Regulation, Neoplastic
• Humans
• Liver Neoplasms (genetics, pathology)
• MicroRNAs (genetics, metabolism)
• Neoplasm Invasiveness
• RNA, Circular (genetics, metabolism)
• Signal Transduction
• Vascular Endothelial Growth Factor A (metabolism)