Preparation of Biodegradable Polymeric Nanocapsules for Treatment of Malignant Tumor Using Coaxial Capillary Microfluidic Device.

Abstract

Objective: Nanocapsules play a role in the targeted delivery of chemotherapy drugs. However, the traditional technology for preparation of nanocapsules is relatively complex with poor controllability, leading to large differences batch to batch. This study aimed to evaluate the quality of drugs-loaded nanocapsules (Drugs-NCs) fabricated by coaxial capillary microfluidic device, and inhibitory effect on malignant tumors. Materials and Methods: In this study, oxaliplatin, irinotecan, and 5-fluorouracil were selected as chemotherapy drugs, and Drugs-NCs were prepared by coaxial glass capillary microfluidic device. Next, transmission electron microscope was utilized to characterize surface morphology and particle size distribution of Drugs-NCs. Then, high performance liquid chromatography was used to determine the drug loading and encapsulation efficiency. Dialysis method was performed to measure the drug release of Drugs-NCs in vitro. To study the effects of Drugs + NCs on tumor growth in vivo, BALB/c (nu/nu) nude mice were used in vivo experiments. Results: The Drugs-NCs were spherical and uniform in size (103.4 nm). Besides, the encapsulation efficiencies of oxaliplatin, irinotecan, and 5-fluorouracil were 97.0%, 95.7%, and 15.6%, respectively. Moreover, drugs encapsulated in the nanocapsules released less and was pH-dependent, with more rapid release being observed at pH 5.5 group compared with pH 7.4 group. MTT assay and in vivo experiments indicated the inhibitory effect of Drugs-NCs on malignant tumors. Conclusion: The prepared nanocapsules had potential tumor targeting. Furthermore, coaxial capillary microfluidic device could be used as a promising microfluidic technology to fabricate multiple Drug-NCs.

• Authors: Yi Xu, Bingren Hu, Jiong Xu, Jianzhang Wu, Bailiang Ye
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 35 Issue 8 Pg. 570-580 (Oct 2020) ISSN: 1557-8852 [Electronic] United States
• PMID: 32196366 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Biodegradable Plastics
• Drug Carriers
• Nanocapsules
• Polymers
• Oxaliplatin
• Irinotecan
• Fluorouracil

Topics

• Animals
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage, pharmacokinetics)
• Biodegradable Plastics (chemistry)
• Cell Line, Tumor
• Drug Carriers (chemistry)
• Drug Compounding (instrumentation, methods)
• Drug Liberation
• Fluorouracil (administration & dosage, pharmacokinetics)
• Humans
• Irinotecan (administration & dosage, pharmacokinetics)
• Lab-On-A-Chip Devices
• Male
• Mice
• Nanocapsules (chemistry)
• Neoplasms (drug therapy, pathology)
• Oxaliplatin (administration & dosage, pharmacokinetics)
• Particle Size
• Polymers (chemistry)
• Reproducibility of Results
• Surface Properties
• Xenograft Model Antitumor Assays