Abstract |
An alkali-soluble, water-soluble cell wall fraction of Blastomyces dermatitidis, designated B-ASWS, was evaluated as an antigen for detecting in vivo (skin tests) and in vitro migration inhibition factor (MIF) production and lymphocyte transformation (LT) responses in Blastomyces-infected guinea pigs. The biological activity of B-ASWS was compared with that of blastomycin KCB-26. The superiority of B-ASWS, in terms of its sensitivity and specificity, was evident in in vivo and in vitro assays. Skin tests responses were obtained in 21 of the 24 Blastomyces-infected guinea pigs, whereas only one of the 14 Histoplasma-infected guinea pigs were significantly greater than those obtained using cell populations from Histoplasma-infected or noninfected guinea pigs. The con-MIF and LT in peritoneal exudate cells and lymph node cells of homologuosly infected animals. In each biological system, the response of the Blastomyces-infected guinea pigs were significantly greater than those obtained using cell populations from Histoplasma-infected or non-infected guinea pigs. The contrasting efficacy of B-ASWS as compared with blastomycin KCB-26, suggests that the cell wall antigen will be a useful tool for detecting cell-mediated immune responses in blastomycosis.
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Authors | F Deighton, R A Cox, N K Hall, H W Larsh |
Journal | Infection and immunity
(Infect Immun)
Vol. 15
Issue 2
Pg. 429-35
(Feb 1977)
ISSN: 0019-9567 [Print] United States |
PMID | 321349
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Fungal
- Macrophage Migration-Inhibitory Factors
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Topics |
- Animals
- Antigens, Fungal
- Blastomyces
(immunology)
- Blastomycosis
(immunology)
- Cell Migration Inhibition
- Cell Wall
(immunology)
- Female
- Guinea Pigs
- Histoplasmosis
(immunology)
- Hypersensitivity, Delayed
- Immunity, Cellular
- Lymphocyte Activation
- Macrophage Migration-Inhibitory Factors
(biosynthesis)
- Male
- Skin Tests
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