Vaccarin hastens wound healing by promoting angiogenesis via activation of MAPK/ERK and PI3K/AKT signaling pathways in vivo.

Abstract

PURPOSE:

To explore the potential role and unclear molecular mechanisms of vaccarin in wound healing.

METHODS:

Rats' skin excision model to study the effects of vaccarin on wound healing in vivo . Hematoxylin and eosin staining was performed to evaluate Histopathologic characteristics. Immunohistochemistry was employed to assess the effects of vaccarin in accelerating angiogenesis. Western blot was used to evaluate relative protein expressed levels.

RESULTS:

Vaccarin could significantly promote wound healing and endothelial cells and fibroblasts proliferation in the wound site. Immunohistochemistry and Western blot studies showed that the nodal proteins and receptor (bFGFR) related to angiogenesis signaling pathway were activated, and the microvascular density in the wound site was markedly higher than that in the control group.

CONCLUSIONS:

The present study was the first to demonstrate that vaccarin is able to induce angiogenesis and accelerate wound healing in vivo by increasing expressions of p-Akt, p-Erk and p-bFGFR. This process is mediated by MAPK/ERK and PI3K/AKT signaling pathways.

• Authors: Bao Hou, Weiwei Cai, Ting Chen, Zhixuan Zhang, Haifeng Gong, Wei Yang, Liying Qiu
• Journal: Acta cirurgica brasileira (Acta Cir Bras) Vol. 34 Issue 12 Pg. e201901202 ( 2020) ISSN: 1678-2674 [Electronic] Brazil
• PMID: 32049183 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Angiogenesis Inducing Agents
• Plant Extracts
• Receptor, Fibroblast Growth Factor, Type 1
• Mitogen-Activated Protein Kinase Kinases

Topics

• Angiogenesis Inducing Agents (pharmacology)
• Animals
• Blotting, Western
• Caryophyllaceae (chemistry)
• Cell Proliferation (drug effects)
• Endothelial Cells (drug effects)
• Fibroblasts (drug effects)
• Immunohistochemistry
• Male
• Mitogen-Activated Protein Kinase Kinases (analysis, drug effects)
• Phosphatidylinositol 3-Kinases (analysis, drug effects)
• Plant Extracts (chemistry, pharmacology)
• Rats, Sprague-Dawley
• Receptor, Fibroblast Growth Factor, Type 1 (analysis, drug effects)
• Reproducibility of Results
• Signal Transduction
• Time Factors
• Wound Healing (drug effects)