Exosome-mediated transfer of miR-1260b promotes cell invasion through Wnt/β-catenin signaling pathway in lung adenocarcinoma.

Abstract

Increasing evidence confirms that exosome-mediated transfer of microRNAs can influence cancer progression including tumor cell invasion, cell proliferation, and drug resistance via cell-cell communication. However, the potential role of exosomal-miR-1260b in lung adenocarcinoma (LAC) remains poorly understood. Thus, this study focused on investigating the function of exosomal-miR-1260b on cell invasion. Exosomal-miR-1260b was found to be higher in plasma of patients with LAC than that of healthy persons via quantitative real-time polymerase chain reaction assay. The sensitivity and specificity of exosomal-miR-1260b (cutoff point: 2.027) were 72% and 86%, and area under the curve of 0.845 (95% CI = 0.772-0.922). Elevated expression of miR-1260b in LAC tissues was positively correlated with exosomal-miR-1260b in plasma (r = .642, p < .05). Furthermore, ceramide biosynthesis regulated exosomal-miR-1260b secretion. Exosome-mediated transfer of miR-1260b promoted A549 cell invasion and was still functional inside A549 cells. Moreover, exosomal-miR-1260b regulated Wnt/β-catenin signaling pathway by inhibiting sFRP1 and Smad4. This study identified a new regulation mechanism involving in cell invasion by exosome-mediated tumor-cell-to-tumor-cell communication. Targeting exosome-microRNAs may provide new insights into the diagnosis and treatment of LAC.

• Authors: Yang Xia, Ke Wei, Liu-Qing Hu, Cun-Rong Zhou, Zhi-Bin Lu, Gen-Sheng Zhan, Xiang-Long Pan, Chun-Feng Pan, Jun Wang, Wei Wen, Jing Xu, Zhi-Cheng He, Chen-Jun Huang, Liang Chen
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 235 Issue 10 Pg. 6843-6853 (10 2020) ISSN: 1097-4652 [Electronic] United States
• PMID: 32026462 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley Periodicals, Inc.

Chemical References

• CTNNB1 protein, human
• Ceramides
• MIRN1260 microRNA, human
• Membrane Proteins
• MicroRNAs
• Smad4 Protein
• beta Catenin

Topics

• A549 Cells
• Adenocarcinoma of Lung (genetics, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Ceramides (genetics)
• Exosomes (genetics)
• Female
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Lung Neoplasms (genetics, pathology)
• Male
• Membrane Proteins (genetics)
• MicroRNAs (genetics)
• Middle Aged
• Signal Transduction (genetics)
• Smad4 Protein (genetics)
• Wnt Signaling Pathway (genetics)
• beta Catenin (genetics)