Abstract |
MIR4435-2HG has been characterized as an oncogenic lncRNA in several types of cancer, while its role in oral squamous cell carcinoma (OSCC, a major subtype of oral cancer) has not been characterized. We explored the functionality of MIR4435-2HG in OSCC and investigated its interactions with TGF-β1. Blood samples were extracted from OSCC patients (n = 44) and healthy volunteers (n = 38), RT-qPCR, CCK-8, Transwell assays and western blot were performed in this study. The results showed that levels of MIR4435-2HG and TGF-β1 in plasma were upregulated in OSCC. Across OSCC plasma samples, TGF-β1 and MIR4435-2HG were significantly and positively correlated. Overexpression of MIR4435-2HG resulted in upregulated TGF-β1 expression, while exogenous TGF-β1 treatment had no effect on the expression of MIR4435-2HG. Overexpression of MIR4435-2HG and exogenous TGF-β1 treatment led to promoted, while TGF-β inhibitor led to inhibited migration, proliferation and invasion of cancer cells. Moreover, TGF-β inhibitor led to reduced effects of overexpressing MIR4435-2HG. Therefore, MIR4435-2HG regulates the behaviors of OSCC cells by promoting the expression of TGF-β1.
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Authors | Huan Shen, Bin Sun, Yongjin Yang, Xingwei Cai, Lixia Bi, Lin Deng, Luyue Zhang |
Journal | Odontology
(Odontology)
Vol. 108
Issue 4
Pg. 553-559
(Oct 2020)
ISSN: 1618-1255 [Electronic] Japan |
PMID | 32016787
(Publication Type: Journal Article)
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Chemical References |
- RNA, Long Noncoding
- Transforming Growth Factor beta1
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Topics |
- Carcinoma, Squamous Cell
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Gene Expression Regulation, Neoplastic
- Humans
- Mouth Neoplasms
(genetics)
- RNA, Long Noncoding
- Transforming Growth Factor beta1
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