Insight the Biological Activities of Selected Abietane Diterpenes Isolated from Plectranthus spp.

Abstract

Natural compounds isolated from plants are excellent starting points in drug design and have been widely studied as anticancer agents; they hence find use in a considerable proportion of anticancer drugs. The genus Plectranthus (Lamiaceae) comprises a large and widespread group of species with various applications in traditional medicine. Therefore, the aim of the present study was to determine the effectiveness of treatment with four abietane diterpenoids isolated from P.madagascariensis and P.ecklonii, 6,7-dehydroroyleanone, 7β,6β-dihydroxyroyleanone, 7α-acetoxy-6β-hydroxyroyleanone and parvifloron D, in initiating apoptosis in a glioma cell line. The pure compounds were found to exhibit anticancer effects in primary H7PX glioma cells line by inducing apoptosis G2/M cell cycle arrest and double-strand breaks, indicated by increased levels of phosphorylated H2A.X and decreasing mitochondrial membrane potential; they also influenced the expression of pro- and anti-apoptotic genes (Bax, Bcl-2, TP53, or Cas-3). Our findings indicate that these compounds may offer potential as beneficial antitumor drugs but further in vivo studies are needed.

• Authors: Przemysław Sitarek, Monika Toma, Epole Ntungwe, Tomasz Kowalczyk, Ewa Skała, Joanna Wieczfinska, Tomasz Śliwiński, Patricia Rijo
• Journal: Biomolecules (Biomolecules) Vol. 10 Issue 2 (01 28 2020) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 32012853 (Publication Type: Journal Article)

Chemical References

• 2-(4-hydroxy)-benzoyloxy-11-hydroxy-5,7,9(11),13-abietatetraen-12-one
• Abietanes
• Antineoplastic Agents, Phytogenic
• Histones
• Plant Extracts

Topics

• Abietanes (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis
• Brain Neoplasms (drug therapy)
• Cell Cycle
• Cell Line, Tumor
• Cell Survival
• DNA Breaks, Double-Stranded
• Drug Design
• Flow Cytometry
• Glioma (drug therapy)
• Histones (chemistry)
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Necrosis
• Phosphorylation
• Plant Extracts (pharmacology)
• Plectranthus (chemistry)