Invasion of TC-1 cells to skeletal muscle fibers protect them from peroxisomicine A1 (T-514) treatment in a murine model of cancer.

Abstract

T-514 or Peroxisomicine A1 (PA1) is a toxin isolated from plants of genus Karwinskia. In vitro studies described selective toxicity of PA1 on malignant cell lines. A toxic effect of PA1 on TC-1 cells in vivo was reported. The objective was to evaluate the effect of PA1 over invasion of TC-1 cells to muscle fibers in vivo. TC-1 cells were implanted in 36 mice divided in two groups (n: 18): treated with PA1 or with vehicle, a control group was included. At 10 days, nine mice of each group were euthanized. TC-1 implant site was analyzed by light and electron microscopy, a morphometric study was also performed. Remaining mice were used to evaluate tumor growth and survival time. Results show tumor cells between muscle fibers, with diminution in diameter, change in the staining pattern, loss of continuity of external lamina, and sarcoplasm with tumor cells. Statistically difference was observed between treated group vs control group. PA1 decreased tumor growth and increased the survival time in treated mice. The degree of resistant activity, aggressiveness, and invasiveness of TC-1 cells described in present work; should be taken into account in studies that evaluate effectiveness of therapies using this cancer model.

• Authors: Odila Saucedo-Cárdenas, Rubén García-Garza, Rosalba Ramírez-Durón, Gerardo E Muñoz-Maldonado, Arnulfo Villanueva-Olivo, Roberto Montes-de-Oca-Luna, Adolfo Soto-Domínguez
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 10 Issue 7 Pg. 8062-8071 ( 2017) ISSN: 1936-2625 [Electronic] United States
• PMID: 31966659 (Publication Type: Journal Article)
• Copyright: IJCEP Copyright © 2017.