Human glucosamine-6-sulfatase cDNA reveals homology with steroid sulfatase.

Abstract

Glucosamine-6-sulfatase is a lysosomal enzyme which degrades glycosaminoglycans and is deficient in mucopolysaccharidosis type IIID. Human liver contains two major active forms of glucosamine-6-sulfatase, form A which has a single 78 kDa polypeptide and form B which has two polypeptides of 48 kDa and 32 kDa. A 1761 base pair cDNA clone encoding the complete 48 kDa polypeptide of form B was isolated. Form A is shown to be processed to form B with the 48 kDa polypeptide C-terminal to the 32 kDa polypeptide, and it is shown that C-terminal processing is limited to a region of thirty amino acids. The glucosamine-6-sulfatase sequence reveals homology with steroid sulfatase, a microsomal enzyme.

• Authors: D A Robertson, C Freeman, P V Nelson, C P Morris, J J Hopwood
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 157 Issue 1 Pg. 218-24 (Nov 30 1988) ISSN: 0006-291X [Print] United States
• PMID: 3196333 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA
• Sulfatases
• Arylsulfatases
• N-acetylglucosamine-6-sulfatase
• Steryl-Sulfatase

Topics

• Amino Acid Sequence
• Arylsulfatases (genetics)
• Base Sequence
• Cloning, Molecular
• DNA (genetics)
• Humans
• Molecular Sequence Data
• Molecular Weight
• Steryl-Sulfatase
• Sulfatases (genetics)