Sodium benzoate inhibits fatty acid oxidation in rat liver: effect on ammonia levels.

Abstract

Sodium benzoate inhibited PC and octanoic acid-mediated State 3 respiration rates by 39 and 29%, respectively, at 0.5 mM in isolated rat liver mitochondria. At 2 mM, benzoate did not affect State 3 respiration rates with either succinate or malate plus glutamate, indicating that it did not act as an uncoupler. The oxidation of palmitate and octanoate was inhibited by 39 and 54% at 2 mM benzoate in liver homogenates. Benzoate, at 10 mmol/kg caused significant decreases in the levels of hepatic ATP, CoA, and acetyl-CoA. Administration of sodium benzoate to rats caused a dose-dependent increase in hepatic ammonia levels. However, the inhibitory effect of benzoate on fatty acid oxidation is not mediated through ammonia since ammonium chloride, at 1 mM, did not inhibit PC or octanoate oxidation in mitochondria or their oxidation in liver homogenate. Our results warrant a reevaluation of the use of sodium benzoate in the treatment of hyperammonemia.

• Authors: S S Kalbag, A G Palekar
• Journal: Biochemical medicine and metabolic biology (Biochem Med Metab Biol) Vol. 40 Issue 2 Pg. 133-42 (Oct 1988) ISSN: 0885-4505 [Print] United States
• PMID: 3190922 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoates
• Caprylates
• Fatty Acids
• Palmitates
• Acetyl Coenzyme A
• Ammonia
• Adenosine Triphosphate
• Benzoic Acid
• Coenzyme A

Topics

• Acetyl Coenzyme A (metabolism)
• Adenosine Triphosphate (metabolism)
• Ammonia (metabolism)
• Animals
• Benzoates (pharmacology)
• Benzoic Acid
• Caprylates (metabolism)
• Coenzyme A (metabolism)
• Fatty Acids (metabolism)
• Liver (drug effects, metabolism)
• Mitochondria, Liver (drug effects, metabolism)
• Oxidation-Reduction
• Oxygen Consumption (drug effects)
• Palmitates (metabolism)
• Rats
• Rats, Inbred Strains