Gallidermin: a new lanthionine-containing polypeptide antibiotic.

Abstract

Gallidermin is a new member of the class of lanthionine-containing peptide antibiotics, which are summarized under the common name lantibiotics. The lantibiotic gallidermin is produced by Staphylococcus gallinarum (F16/P57) Tü3928, and it exhibits activities against the Propionibacteria, involved in acne disease. Gallidermin differs from the recently discovered tetracyclic 21-residue peptide antibiotic epidermin only in a Leu/Ile exchange in position 6. The isolation procedures for gallidermin included adsorption directly from the culture broth, ion-exchange chromatography of the amphiphilic and basic polypeptide followed by desalting, and final purification by reversed-phase HPLC. The structural elucidation of the polypeptide containing four thioether bridges involved mainly a combination of automated gas-phase sequencing, thermospray liquid chromatography/mass spectrometry and fast-atom-bombardment mass spectrometry.

• Authors: R Kellner, G Jung, T Hörner, H Zähner, N Schnell, K D Entian, F Götz
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 177 Issue 1 Pg. 53-9 (Oct 15 1988) ISSN: 0014-2956 [Print] England
• PMID: 3181159 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Bacteriocins
• Peptides
• Peptides, Cyclic
• Sulfides
• gallidermin
• epidermin
• lanthionine
• Alanine

Topics

• Alanine (analogs & derivatives, analysis)
• Amino Acid Sequence
• Anti-Bacterial Agents (isolation & purification, pharmacology)
• Bacteriocins
• Chromatography, High Pressure Liquid
• Chromatography, Ion Exchange
• Gas Chromatography-Mass Spectrometry
• Microbial Sensitivity Tests
• Molecular Sequence Data
• Peptides
• Peptides, Cyclic (isolation & purification, pharmacology)
• Staphylococcus (metabolism)
• Sulfides