Massive clonal expansion of medulloblastoma-specific T cells during adoptive cellular therapy.
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| Abstract |
In both human and murine systems, we have developed an adoptive cellular therapy platform against medulloblastoma and glioblastoma that uses dendritic cells pulsed with a tumor RNA transcriptome to expand polyclonal tumor-reactive T cells against a plurality of antigens within heterogeneous brain tumors. We demonstrate that peripheral TCR Vβ repertoire analysis after adoptive cellular therapy reveals that effective response to adoptive cellular therapy is concordant with massive in vivo expansion and persistence of tumor-specific T cell clones within the peripheral blood. In preclinical models of medulloblastoma and glioblastoma, and in a patient with relapsed medulloblastoma receiving adoptive cellular therapy, an early and massive expansion of tumor-reactive lymphocytes, coupled with prolonged persistence in the peripheral blood, is observed during effective therapeutic response to immunotherapy treatment.
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| Authors | C Flores, T Wildes, B DiVita Dean, G Moore, J Drake, R Abraham, J Gil, O Yegorov, C Yang, J Dean, C Moneypenny, D Shin, C Pham, J Krauser, J King, G Grant, T Driscoll, J Kurtzberg, R McLendon, S Gururangan, D Mitchell |
| Journal | Science advances (Sci Adv) Vol. 5 Issue 11 Pg. eaav9879 (11 2019) ISSN: 2375-2548 [Electronic] United States |
| PMID | 31807694 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). |
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