[DESIGN AND EVALUATION OF A MULTIEPITOPIC PROTEIN AS A CANDIDATE FOR A CARRION DISEASE VACCINE].

Abstract

OBJECTIVES.:

To design and assess a multiepitopic protein as a candidate for a vaccine against Carrion disease.

MATERIALS AND METHODS.:

Using bioinformatics tools, epitopes of external membrane proteins were selected and a multiepitopic protein was designed. The multiepitopic protein gene was subcloned into the expression plasmid pET28b and transformed into E. coli BL21 pLys. The multiepitopic protein was expressed using isopropyl-β-D-1-thiogalactopyranoside and purified using resin. This purified protein was used to immunize BALB/c mice obtaining polyclonal antibodies. In vitro invasion assays were conducted using a strain of Bartonella bacilliformis (B. bacilliformis) in human red blood cells.

RESULTS.:

The multiepitopic protein M1 presents preserved epitopes between isolates of B. bacilliformis with are non-toxic, and not homologous to human and surface proteins. Immunized mice presented IgG antibody levels capable of reducing in vitro the rate of invasion of B. bacilliformis into human red blood cells.

CONCLUSIONS.:

Multiepitopic protein M1 may serve as a candidate for a Carrion disease vaccine; however, more studies are needed to characterize the use of this antigen as a vaccine.

• Authors: Carlos Patricio Padilla Rojas, Priscila Nayu Lope Pari, Lorena Santos Solis, Cleidy Osorio Mogollón, Lisbet Inga Angulo, Henri Bailon Calderon, Adolfo Marcelo Ñique, Jackeline Morales, Gladis Esther Ventura Egusquiza
• Journal: Revista peruana de medicina experimental y salud publica (Rev Peru Med Exp Salud Publica) 2019 Jul-Sep Vol. 36 Issue 3 Pg. 414-422 ISSN: 1726-4642 [Electronic] Peru
• Vernacular Title: DISEÑO Y EVALUACIÓN DE UNA PROTEÍNA MULTIEPITÓPICA COMO CANDIDATA PARA VACUNA CONTRA LA ENFERMEDAD DE CARRIÓN.
• PMID: 31800933 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Bacterial Vaccines
• Epitopes

Topics

• Animals
• Bacterial Proteins (biosynthesis)
• Bacterial Vaccines (biosynthesis)
• Bartonella Infections (prevention & control)
• Computational Biology
• Drug Design
• Epitopes
• Female
• Mice, Inbred BALB C