Antibody to mouse interferon alpha/beta abrogates resistance to the multiplication of Friend erythroleukemia cells in the livers of allogeneic mice.

Abstract

Friend erythroleukemia cells (FLC) (H-2d) injected intravenously into adult syngeneic DBA/2 or allogeneic C57B1/6 (H-2b) or C3H (H-2k) mice lodge in the liver but only multiply in the liver of syngeneic mice. Our results indicated that endogenous IFN-alpha/beta was a crucial factor in preventing the multiplication of FLC in the liver of adult allogeneic mice. (a) Treatment of allogeneic adult C57B1/6 or C3H mice with polyclonal antibody to mouse IFN-alpha/beta (but not antibody to IFN-gamma) completely abrogated the resistance to the multiplication of FLC in the liver and 87% of tumor-injected, antibody-treated C57B1/6 mice died with extensive tumor involvement of the liver. In contrast, after intravenous inoculation FLC do not multiply at all (or very rarely) in the liver of adult C57B1/6 mice left untreated or treated with a variety of control globulins, and no deaths occurred. (b) 8 h after intravenous inoculation of FLC, poly(A)+ RNA hybridizable with specific DNA probes for mouse IFN-alpha or -beta (but not -gamma) was present in the liver of injected C57B1/6 mice. Using the expression of the Mx protein as an indicator of the presence of IFN-alpha/beta, we showed that Mx+ congenic C57B1/6 mice injected with FLC exhibited a marked increase in the expression of the Mx protein in the liver, spleen, kidney and lung, and this increase was blocked by treatment of mice with antibody to IFN-alpha/beta. The possibility that different host mechanisms are elicited depending on the site of tumor growth in allogeneic mice is discussed. IFN-alpha/beta appears to be of particular importance in determining the resistance of the liver to FLC in allogeneic mice.

• Authors: I Gresser, C Maury, F Vignaux, O Haller, F Belardelli, M G Tovey
• Journal: The Journal of experimental medicine (J Exp Med) Vol. 168 Issue 4 Pg. 1271-91 (Oct 01 1988) ISSN: 0022-1007 [Print] United States
• PMID: 3171480 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Antibodies, Monoclonal
• Antiviral Agents
• DNA Probes
• Interferon Type I
• Mx1 protein, mouse
• Myxovirus Resistance Proteins
• Proteins
• RNA, Messenger
• Serum Globulins
• GTP-Binding Proteins

Topics

• Age Factors
• Animals
• Antibodies (immunology)
• Antibodies, Monoclonal (immunology)
• Antiviral Agents (genetics, immunology)
• DNA Probes
• Friend murine leukemia virus
• GTP-Binding Proteins
• Interferon Type I (immunology)
• Leukemia, Erythroblastic, Acute (immunology, pathology)
• Liver Neoplasms, Experimental (immunology, pathology)
• Major Histocompatibility Complex
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Myxovirus Resistance Proteins
• Neoplasm Transplantation
• Proteins (genetics, immunology)
• RNA, Messenger (analysis)
• Serum Globulins (immunology)
• Tumor Cells, Cultured