Tissue samples from 103 patients with gallbladder
carcinoma were examined, using 5-mm stepwise tissue sections. Three pathologic stages were used: Stage I;
carcinoma invading not further than the muscle coat of the gallbladder, with or without extension along Rokitansky-Aschoff sinuses in the subserosa (11 cases); Stage II,
carcinoma extending to the subserosal fibroadipose tissue of the gallbladder (73 cases); and Stage III:
carcinoma invading the adjacent organs such as the duodenum, liver, and colon (19 cases). In the 11 patients with Stage I
carcinoma, there was no apparent
lymph node metastasis and all remained well for 3 months to 14 years after the initial operation. The cumulative 3-year survival rate of Stage I (100%) was significantly higher than of Stage II (40%) and Stage III (10%) (P less than 0.01, P less than 0.001). The Stage I
tumors, therefore, can be defined as early
carcinoma of the gallbladder. Dysplastic epithelium was seen in the mucosa adjacent to the malignant lesion in 57% of those with gallbladder
carcinoma: 73% in Stage I, 59% in Stage II, and 42% in Stage III. Nine gallbladders had multiple foci of
adenocarcinoma in a background of a diffuse dysplasia. Immunohistochemical study for
carcinoembryonic antigen and
carbohydrate antigen 19-9 resulted in positive staining of the dysplastic epithelial
element adjacent to the invasive
carcinoma and, in a similar fashion, in the
carcinoma itself, thereby indicating a close relation between the epithelial dysplasia and
adenocarcinoma of the gallbladder.