Effect of Periapical Diseases in Development of MRONJ in Immunocompromised Mouse Model.

Abstract

OBJECTIVES:

This study aimed to assess the effect of zoledronic acid on an immunocompromised mice model with periapical disease.

MATERIALS AND METHODS:

Thirty C57BL/6N mice were randomly divided into three groups (N = 10). All animals were subjected to bilateral ovariectomy (OVX) and then treated with saline (Veh), zoledronic acid (ZA), or concomitant zoledronic acid and dexamethasone (ZA/Dx) for 12 weeks. Eight weeks after starting drug administration, pulpal exposure was conducted on the lower left first molar. Four weeks after pulpal exposure, all mice were sacrificed and the mandibles were collected for radiological and histological examinations.

RESULTS:

Microcomputed tomography (μ-CT) examination showed significantly reduced periapical bone resorption in the ZA/Dx group and decreased periodontal bone resorption in both ZA and ZA/Dx groups. Higher bone mineral density (BMD) and strengthened microstructure were found in ZA and ZA/Dx groups. More empty lacunae were found in ZA and ZA/Dx groups.

CONCLUSIONS:

Apical periodontitis aggravates MRONJ under immunocompromised circumstances. Concurrent use of ZA and steroids inhibits alveolar bone resorption but increases the risk of developing MRONJ.

• Authors: Nian Jing Rao, Ru Qing Yu, Jing Yi Wang, Alexandra Helm, Li Wu Zheng
• Journal: BioMed research international (Biomed Res Int) Vol. 2019 Pg. 1271492 ( 2019) ISSN: 2314-6141 [Electronic] United States
• PMID: 31662968 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Nian Jing Rao et al.

Chemical References

• Bone Density Conservation Agents
• Zoledronic Acid
• Dexamethasone

Topics

• Alveolar Bone Loss (diagnostic imaging, drug therapy, pathology)
• Animals
• Bone Density (drug effects)
• Bone Density Conservation Agents (therapeutic use)
• Dexamethasone (pharmacology)
• Disease Models, Animal
• Female
• Mandible (drug effects)
• Mice
• Mice, Inbred C57BL
• Molar (drug effects)
• Osteonecrosis (drug therapy)
• Ovariectomy
• Periapical Diseases (diagnostic imaging, drug therapy, pathology)
• X-Ray Microtomography
• Zoledronic Acid (therapeutic use)