Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Abstract

Topical management of oral infection requires combined use of multiple classes of drugs and frequent dosing due to low drug retention rates. The sustained, co-delivery of drugs with different solubilities to cells using nanoparticle drug delivery systems remains a challenge. Here, we developed wheat germ agglutinin (WGA) conjugated liposomes with surface grafted cyclodextrin (WGA-liposome-CD) as bioadhesive dual-drug nanocarriers. We effectively encapsulated two physiochemically different drugs (ciprofloxacin and betamethasone) and demonstrated sustained co-drug release in saliva over a 24 h period in vitro. As proof of therapeutic utility in oral cells, we infected oral keratinocytes with Aggregatibacter actinomycetemcomitans, a bacterial pathogen responsible for chronic periodontal disease. Drug release, resulting from nanocarrier cell binding, produced a significant increase in oral cell survival and synergistically reduced inflammation. These results suggest that WGA-liposome-CD nanocarriers are novel cyto-adhesive candidates for delivering multiple drugs with sustained therapeutic activity for localized drug delivery to oral cells.

• Authors: Sashini S Wijetunge, Jianchuan Wen, Chih-Ko Yeh, Yuyu Sun
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 185 Pg. 110572 (Jan 01 2020) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 31654890 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Adhesives
• Cyclodextrins
• Drug Carriers
• Liposomes
• Wheat Germ Agglutinins
• Nitric Oxide

Topics

• Adhesives (pharmacology)
• Aggregatibacter (drug effects)
• Animals
• Cattle
• Cell Death
• Cell Line
• Cyclodextrins (pharmacology)
• Drug Carriers (chemistry)
• Drug Delivery Systems
• Drug Liberation
• Humans
• Liposomes
• Mouth (cytology, microbiology)
• Nanoparticles (chemistry)
• Nitric Oxide (biosynthesis)
• Wheat Germ Agglutinins (pharmacology)