Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection.

Abstract

Interleukin-10 (IL-10) is a pleiotropic cytokine and plays a crucial role in immunity. In the current study, we examined the expression patterns and biological functions of tongue sole Cynoglossus semilaevis IL-10 (CsIL-10). CsIL-10 is composed of 186 amino acid residues and shares 46.3%-71.7% identities with other teleost IL-10. Csil-10 expression occurred in multiple tissues and was regulated by bacterial infection. Recombinant CsIL-10 (rCsIL-10) in the form of a dimer bound to a wide range of bacterial species but did not affect bacterial growth. rCsIL-10 could interact with peripheral blood leukocytes (PBL) and significantly reduce the phagocytic activity, ROS production, and apoptosis of PBL. When injected in vivo, rCsIL-10 significantly suppressed the expression of proinflammatory cytokines and promoted bacterial dissemination in tongue sole tissues. Consistently, knockdown of Csil-10 significantly inhibited bacterial infection in tongue sole. Taken together, these results indicate that CsIL-10 plays a negative regulatory role in the immune response against bacterial infection.

• Authors: Xue-Peng Li, Shuai Jiang, Bin Sun, Jian Zhang
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 95 Pg. 93-104 (Dec 2019) ISSN: 1095-9947 [Electronic] England
• PMID: 31618681 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Fish Proteins
• Recombinant Proteins
• Interleukin-10

Topics

• Animals
• Bacteria
• Bacterial Infections (immunology, veterinary)
• Fish Diseases (immunology, microbiology)
• Fish Proteins (immunology)
• Flatfishes (immunology, microbiology)
• Immunity, Innate
• Interleukin-10 (immunology)
• Phagocytosis
• Recombinant Proteins (immunology)