Development of a disposable micro-capillary film grafted with peptide ligands for immunoadsorption.

Abstract

Traditional chromatographic techniques used in downstream processes of biomolecule manufacturing are often time-consuming and expensive. In this study, a cost-effective microporous micro-capillary film (MMCF) composed of ethylenevinyl alcohol (EVOH) was evaluated for its potential application in immunoadsorption with high process efficiency. A peptide ligand Ac-Phe-Tyr-His-Glu (Ac-FYHE) was immobilized on the inner surface of MMCF for selective binding of human immunoglobulin (hIgG). The porous structure and chemical properties of the prepared MMCF were confirmed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). hIgG (2 mg/ml) adsorption studies demonstrated that the binding process followed a Langmuir isotherm with equilibrium adsorption capacities of 9.31 and 3.47 mg/ml adsorbent under static and dynamic conditions, respectively. Moreover, the membrane showed good flowrate tolerance when studied under flowrates of 0.5 ml/min to 10 ml/min. hIgG purity was 88.2% when obtained from an hIgG (2 mg/ml) and HSA (8 mg/ml) mixture and the purity remained over 80.0% when hIgG concentrations increased in the mixtures. Moreover, purity of 82.3% was achieved when removing hIgG directly from human serum. The MMCF-Ac-FYHE affinity column is expected to selectively remove hIgG from blood for the treatment of autoimmune diseases with high efficiency and cost effectiveness.

• Authors: Jiawen Li, Dongqiang Lin, Shanjing Yao, N K H Slater, Qilei Zhang
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 1129 Pg. 121801 (Oct 15 2019) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 31610481 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Immunoglobulin G
• Ligands
• Peptides
• Vinyl Compounds
• Serum Albumin, Human

Topics

• Adsorption
• Chromatography, Affinity (methods)
• Humans
• Immunoglobulin G (isolation & purification)
• Kinetics
• Ligands
• Molecular Structure
• Peptides (chemistry)
• Porosity
• Serum Albumin, Human (chemistry)
• Structure-Activity Relationship
• Surface Properties
• Vinyl Compounds (chemistry)