Abstract |
A simple test for studying in vitro Fc-receptor function of mononuclear phagocytes is described. Immune phagocytosis is analyzed as a dynamic phenomenon by using nearly pure suspensions of monocytes incubated for diverse times with autologous erythrocytes sensitized with highly purified IgG. In a series of normal volunteers and patients with vasculitis a strict correlation has been found between this in vitro assay and the measure of splenic clearance of IgG-coated red blood cells (RBC), the classical approach for studying in vivo macrophage Fc-receptor function by using sodium chromate 51Cr as tag. The use of this in vitro assay appears to be valuable mainly in cases requiring repeated measurements of Fc-receptor function for monitoring the course of disease or the effects of therapy.
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Authors | D Roccatello, J C Bensa, R Coppo, C Rollino, M Diaz, G Martina, G Piccoli |
Journal | Immunology letters
(Immunol Lett)
Vol. 9
Issue 1
Pg. 53-6
( 1985)
ISSN: 0165-2478 [Print] Netherlands |
PMID | 3157644
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Chromium Radioisotopes
- Immunoglobulin G
- Receptors, Fc
- Receptors, IgG
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Topics |
- Autoimmune Diseases
(immunology)
- Chromium Radioisotopes
- Erythrocyte Aging
- Erythrocytes
(physiology)
- Humans
- Immunoglobulin G
(metabolism)
- Monocytes
(immunology, metabolism)
- Phagocytosis
- Receptors, Fc
(physiology)
- Receptors, IgG
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