MiR-144-3p Inhibits BMSC Proliferation and Osteogenic Differentiation Via Targeting FZD4 in Steroid-Associated Osteonecrosis.

Abstract

BACKGROUND:

MicroRNAs have recently been recognized to be engaged in the development of bone diseases.

OBJECTIVE:

This study was performed to elucidate the effects of miR-144-3p on proliferation and osteogenesis of mesenchymal stem cells (MSCs) from the patients with steroid-associated osteonecrosis (ONFH) and its related mechanism.

METHOD:

The expression level of miR-144-3p in the MSCs from the proximal femur of the patients was examined by Real-time PCR. The cell proliferation ability was assayed by MTT. The differentiation ability of MSCs was assayed by Alizarin Red S (ARS) staining. The interaction between miR-144-3p and frizzled4 (FZD4) was investigated by Real-time PCR, western blot and luciferase reporter assay.

RESULTS:

ONFH samples had the obviously high expression of miR-144-3p compared to the control. MiR-144-3p had a negative effect on the proliferation and osteogenesis of MSCs. Via targeting FZD4, miR-144-3p decreased β-catenin nuclear translocation, the transcription of RUNX2 and COL1A1. Over-expression of FZD4 partially reversed miR-144-3p-induced decrease in the proliferation and osteogenesis of MSCs.

CONCLUSION:

MiR-144-3p might play an important role in the development of ONFH and might be used as a novel class of therapeutic targets for this disease.

• Authors: Zhibo Sun, Fei Wu, Yue Yang, Feng Liu, Fengbo Mo, Jin Chen, Guangyong Wang, Bo Zhang
• Journal: Current pharmaceutical design (Curr Pharm Des) Vol. 25 Issue 45 Pg. 4806-4812 ( 2019) ISSN: 1873-4286 [Electronic] United Arab Emirates
• PMID: 31566128 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• FZD4 protein, human
• Frizzled Receptors
• MIRN144 microRNA, human
• MicroRNAs
• Steroids

Topics

• Bone Marrow Cells (cytology)
• Cell Differentiation
• Cell Proliferation
• Frizzled Receptors (metabolism)
• Humans
• Mesenchymal Stem Cells (cytology)
• MicroRNAs (metabolism)
• Osteogenesis
• Osteonecrosis (chemically induced, physiopathology)
• Steroids (adverse effects)