Abstract | OBJECTIVE: METHODS: RESULTS: Among all mature B-cell lymphomas, the sensitivity of Ig gene rearrangement was 91.18% (248/272), IgH and IgK gene rearrangement was 76.47% (208/272) and 75.00% (204/272), respectively, meanwhile the sensitivity of TCRγ rearrangement was 3.68% (10/272). In the 55 cases of mature T-cell lymphoma, the sensitivity of the detection of TCRγ was 76.36% (44/55), at the same time the sensitivity of Ig gene rearrangement was 14.55% (8/55), IgH and IgK gene rearrangement was 7.27% (4/55) and 12.73% (7/55), respectively. In 21 cases of extranodal NK/T cell lymphoma, nasal type, and 20 cases of reactive lymphoid hyperplasia, no gene rearrangement was found in the samples of IgH, IgK and TCR. The sensitivity of gene rearrangement in Ig/TCR in B and T-cell lymphoma was significantly different from that in the control group (P < 0.05). CONCLUSION: The Ig/TCR gene rearrangement of BIOMED-2 multiplex PCR strategy has important auxiliary value in the diagnosis of B/T-cell non-Hodgkin lymphoma respectively, however, a few B-cell lymphomas may company TCR gene rearrangement as well as a few T-cell lymphomas may accompany Ig gene rearrangement, it must be comprehensively judged with the combination of morphology, immunohistochemistry and clinical features.
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Authors | Xueni Liu, Hong He, Yuanxin Li, Ying Huang, Gang Li, Qiubo Yu, Wenwen Li, Dan Li |
Journal | Pathology, research and practice
(Pathol Res Pract)
Vol. 215
Issue 11
Pg. 152615
(Nov 2019)
ISSN: 1618-0631 [Electronic] Germany |
PMID | 31562020
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier GmbH. All rights reserved. |
Chemical References |
- Immunoglobulin Heavy Chains
- Immunoglobulin kappa-Chains
- Receptors, Antigen, T-Cell, gamma-delta
|
Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Child
- China
- Female
- Gene Rearrangement
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
(genetics)
- Immunoglobulin kappa-Chains
(genetics)
- Lymphoma, Non-Hodgkin
(diagnosis, genetics)
- Male
- Middle Aged
- Multiplex Polymerase Chain Reaction
(methods)
- Receptors, Antigen, T-Cell, gamma-delta
(genetics)
- Sensitivity and Specificity
- Young Adult
|