Inhibition of inflammatory-molecule synthesis in THP-1 cells stimulated with phorbol 12-myristate 13-acetate by brefelamide derivatives.

Abstract

Plasma osteopontin (OPN) levels are elevated in tuberculosis patients and may involve granuloma formation. New inhibitors using brefelamide, an aromatic amide isolated from Dictyostelium cellular slime molds that may inhibit OPN transcription in A549 cells at 1 μM concentration, were synthesized as compounds C, D, and E. Their inhibitory activity against OPN synthesis in phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 cells was confirmed using enzyme-linked immunosorbent assay (ELISA), a multicolor immune-fluorescent microscope, and western blot. In the ELISA performed using full-length OPN, each compound showed significant inhibition in culture supernatants with half maximal inhibitory concentration (IC50) values of 1.6, 1.8, and 2.2 μM for C, D, and E, respectively. In another ELISA to detect the immune-related form of OPN, IC50 values were 0.6, 1.2, and 2.5 μM for compounds C, D, and E, respectively. The decreases in OPN expression and synthesis were confirmed using immunofluorescence and western blot studies using compound-treated cells or cell lysates. Luminex assay of the supernatants of PMA-treated THP-1 cells showed significant reduction in the synthesis of interleukin (IL)-1β, galectin-9, and tumor necrosis factor (TNF)-α. Elucidation of the detailed mechanisms of the biological activities of these compounds would be necessary; however, they may be used in clinical trials for infectious diseases, inflammatory disorders, and cancer.

• Authors: Gaowa Bai, Takashi Matsuba, Haruhisa Kikuchi, Haorile Chagan-Yasutan, Hirotoshi Motoda, Ryo Ozuru, Osamu Yamada, Yoshiteru Oshima, Toshio Hattori
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 75 Pg. 105831 (Oct 2019) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 31437790 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Amides
• Anti-Inflammatory Agents
• Cytokines
• Galectins
• LGALS9 protein, human
• Phenols
• brefelamide
• Tetradecanoylphorbol Acetate

Topics

• A549 Cells
• Amides (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Cytokines (immunology)
• Galectins (immunology)
• Humans
• Phenols (pharmacology)
• THP-1 Cells
• Tetradecanoylphorbol Acetate