Antitumor effector mechanism at a distant site in the double grafted tumor system of PSK, a protein-bound polysaccharide preparation.

Abstract

The antitumor effect at a distant site of PSK, a Coriolus preparation, was analyzed with the double grafted tumor system in which BALB/c mice received simultaneous intradermal inoculations of Meth-A tumor in the right (10(6) cells) and left (2 x 10(5) cells) flanks and were then injected with PSK in the right-flank tumor on day 3. PSK inhibited the growth of not only the right but also the left (non-treated) tumor. Immunized spleen cells were taken from mice which had been cured by the intratumoral administration of 5 mg of PSK and were injected into the Meth-A tumor on day 3. Adoptive transfer of PSK immunized spleen cells caused the complete regression of Meth-A tumors. The effector cell activity was lost only after treatment with anti-Lyt-1 monoclonal antibody plus complement. Spleen cells and right and left regional lymph node cells prepared from PSK immunized mice were examined for Thy-1, Lyt-1, Lyt-2 and asialo GM1 phenotypes. The number of Lyt-1-positive lymphocytes increased in the right regional lymph nodes after intratumoral administration of PSK. A massive accumulation of macrophages and polymorphonuclear leukocytes was found in the right tumor and an infiltration of macrophages and Lyt-2-positive lymphocytes was found in the left (non-treated) tumor by immunohistochemical analyses. These results suggest that intratumoral administration of PSK induces Lyt-1-positive cells first in regional lymph nodes, then in the spleen, and subsequently induces macrophages and Lyt-2-positive cells in the left (non-treated) tumor, thus bringing about the regression of metastatic tumors.

• Authors: T Ebina, H Kohya
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 79 Issue 8 Pg. 957-64 (Aug 1988) ISSN: 0910-5050 [Print] Japan
• PMID: 3141330 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Antibodies, Monoclonal
• Antigens, Surface
• Proteoglycans
• polysaccharide-K
• Complement System Proteins

Topics

• Animals
• Antibiotics, Antineoplastic (therapeutic use)
• Antibodies, Monoclonal
• Antigens, Surface (analysis)
• Cell Survival
• Complement System Proteins (physiology)
• Flow Cytometry
• Immunohistochemistry
• Lymph Nodes (cytology, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Proteoglycans (therapeutic use)
• Spleen (cytology, immunology)