Stimulation of tumor cell growth in humans by a mononuclear cell-derived factor.
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| Abstract |
Serum-free supernatants of peripheral blood mononuclear cell cultures significantly stimulated [3H]thymidine incorporation of human hematopoietic and nonhematopoietic tumor cell lines. For assay we used a low number of tumor cells per well and medium enriched with dithiothreitol-treated fetal calf serum. The growth-stimulatory activity was detected in the supernatant of peripheral blood mononuclear cell culture within the first 24 h and decreased thereafter. Treatment of mononuclear cells with OKT3 monoclonal antibodies and rabbit complement decreased only moderately the factor production while treatment with anti-Leu-M2 and rabbit complement decreased it significantly. Supernatants of concanavalin A-stimulated peripheral blood mononuclear cell cultures enhanced nonsignificantly [3H]thymidine incorporation by tumor cell cultures unless antibodies against tumor necrosis factor alpha and gamma-interferon were added to the supernatants. Growth-stimulatory activity was heat inactivated partially at 60 degrees C and totally at 80 degrees C. It was abolished at pH 2.5 within 2 h as well as by treatment with dithiothreitol and partially lost by dialysis.
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| Authors | G Sandru, P Veraguth, B M Stadler |
| Journal | Cancer research (Cancer Res) Vol. 48 Issue 19 Pg. 5411-6 (Oct 01 1988) ISSN: 0008-5472 [Print] United States |
| PMID | 3138023 (Publication Type: Journal Article) |
| Chemical References |
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