ω-3 fish oil fat emulsion preconditioning mitigates myocardial oxidative damage in rats through aldehydes stress.

Abstract

ω-3 fish oil fat emulsions contain a considerable quantity of unsaturated carbon-carbon double bonds, which undergo lipid peroxidation to yield low-dose aldehydes. These aldehydes may stimulate the production of antioxidant enzymes, thereby mitigating myocardial oxidative damage. This study aims to (1) verify the cardioprotective effect of ω-3 fish oil fat emulsion in vivo and in vitro, and (2) determine whether aldehyde stress is a protective mechanism. For modeling purposes, we pretreated rats with 2 ml/kg of a 10% ω-3 fish oil fat emulsion for 5 days in order to generate a sufficient aldehyde stress response to trigger the production of antioxidant enzymes, and we obtained similar response with H9C2 cells that were pretreated with a 0.5% ω-3 fish oil fat emulsion for 24 h. ω-3 fish oil fat emulsion pretreatment in vivo reduced the myocardial infarct size, decreased the incidence of arrhythmias, and promoted the recovery of cardiac function after myocardial ischemia/reperfusion injury. Once the expression of nuclear factor E2-related factor 2 (Nrf2) was silenced in H9C2 cells, aldehydes no longer produced enough antioxidant enzymes to reverse the oxidative damage caused by tert-butyl hydroperoxide (TBHP). Our results demonstrated that ω-3 fish oil fat emulsion enhanced the inhibition of oxidation and production of free radicals, and alleviated myocardial oxidative injury via activation of the Nrf2 signaling pathway.

• Authors: Jiaojiao Dong, Xiaona Feng, Jingxiong Zhang, Yujian Zhang, Fangfang Xia, Le Liu, Zhousheng Jin, Caijiao Lu, Yun Xia, Thomas J Papadimos, Xuzhong Xu
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 118 Pg. 109198 (Oct 2019) ISSN: 1950-6007 [Electronic] France
• PMID: 31336342 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Aldehydes
• Antioxidants
• Fatty Acids, Omega-3
• Fish Oils
• Gabpa protein, rat
• NF-E2-Related Factor 2

Topics

• Animals
• Male
• Aldehydes (metabolism)
• Antioxidants (metabolism)
• Cell Line
• Fatty Acids, Omega-3 (administration & dosage, pharmacology)
• Fish Oils (administration & dosage, pharmacology)
• Lipid Peroxidation (drug effects)
• Myoblasts, Cardiac (drug effects, metabolism)
• Myocardial Infarction (metabolism, prevention & control)
• Myocardial Reperfusion Injury (metabolism, prevention & control)
• Rats, Sprague-Dawley
• NF-E2-Related Factor 2 (metabolism)