Alteration of pulmonary structure by Pseudomonas aeruginosa exoenzyme S.

Abstract

Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, fibrinous exudation and released type II cell lamellar bodies in alveolar lumina after 1-12 h; progressively increasing accumulations of polymorphonuclear leucocytes in the bronchi and alveoli and in alveolar septae (interstitial pneumonia) after 1-12 h; collapse of alveolar septal connective tissue and damage to pulmonary arterioles and venules. Treatment of monolayer cultures of bronchial fibroblasts with purified exoenzyme S elicited vacuolation of the cells with apparent membrane damage as revealed by light and electronmicroscopy. In-vivo production and activity of P. aeruginosa exoenzyme S may be an important pathogenicity determinant in the necrotising lung injury characteristic of P. aeruginosa pneumonia.

• Authors: D E Woods, W S Hwang, M S Shahrabadi, J U Que
• Journal: Journal of medical microbiology (J Med Microbiol) Vol. 26 Issue 2 Pg. 133-41 (Jun 1988) ISSN: 0022-2615 [Print] England
• PMID: 3133480 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• ADP Ribose Transferases
• Poly(ADP-ribose) Polymerases
• exoenzyme S

Topics

• ADP Ribose Transferases
• Animals
• Bacterial Toxins
• Bronchi (drug effects, ultrastructure)
• Epithelium (drug effects, ultrastructure)
• Fibroblasts (drug effects, ultrastructure)
• Lung (drug effects, ultrastructure)
• Male
• Microscopy, Electron
• Poly(ADP-ribose) Polymerases (administration & dosage, toxicity)
• Pseudomonas aeruginosa (enzymology, pathogenicity)
• Pulmonary Alveoli (drug effects, ultrastructure)
• Rats
• Rats, Inbred Strains