Lung adenocarcinoma is a common histologic type of
lung cancer with a high death rate globally. Increasing evidence shows that
long non-coding RNA H19 (
lncRNA H19) and CDH1 methylation are involved in multiple tumours. Here, we tried to investigate whether
lncRNA H19 or CDH1 methylation could affect the development of
lung adenocarcinoma. First,
lung adenocarcinoma tissues were collected to detect CDH1 methylation. Then, the regulatory mechanisms of
lncRNA H19 were detected mainly in concert with the treatment of overexpression of
lncRNA H19,
siRNA against
lncRNA H19, overexpression of CDH1 and demethylating agent A-5az in
lung adenocarcinoma A549 cell. The expression of
lncRNA H19 and epithelial-mesenchymal transition (EMT)-related factors as well as cell proliferation, sphere-forming ability, apoptosis, migration and invasion were detected. Finally, we observed xenograft tumour in nude mice so as to ascertain tumorigenicity of
lung adenocarcinoma cells.
LncRNA H19 and methylation of CDH1 were highly expressed in
lung adenocarcinoma tissues. A549 cells with silencing of
lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation by demethylating agent 5-Az had suppressed cell proliferation, sphere-forming ability, apoptosis, migration and invasion, in addition to inhibited EMT process. Silencing
lncRNA H19 could reduce methylation level of CDH1. In vivo, A549 cells with silencing
lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation exhibited low tumorigenicity, reflected by the smaller tumour size and lighter tumour weight. Taken together, this study demonstrates that silencing of
lncRNA H19 inhibits EMT and proliferation while promoting apoptosis of
lung adenocarcinoma cells by inhibiting methylation of CDH1 promoter.