Differential modification of specificity in carbonic anhydrase catalysis.

Abstract

Incubation of carbonic anhydrase II with acrolein results in a rapid, time-dependent loss of all but approximately 3-6% of the original catalytic activity toward CO2 hydration and HCO3- dehydration, with the inactivation rate being first-order in both acrolein and the enzyme. The pH dependence of the inactivation rate constant can be adequately described with a function incorporating a pK alpha of 7.15 and a maximal value for kinact [corrected] of 26.2 M-1 min-1, indicating that at least one of the catalytically essential residues that ionizes at this pH is involved in the modification scheme. The amount of residual CO2 hydratase activity is proportional to the molar excess of acrolein over carbonic anhydrase II with 5 histidyl and 3 lysyl residues being subject to alkylation under conditions where [acrolein] to [carbonic anhydrase II] ratio is greater than 100. Because all lysyl residues were shown previously to be amidinated without detectable loss of activity, it was assumed that the modification of one (or more) of the histidines was primarily responsible for the observed inactivation. The number of modified histidyl residues could be related to residual activity by using the statistical analysis of Tsou (Tsou, C.-L. (1962) Sci. Sin. (Engl. Ed.) 11, 1535-1558) which indicates that one essential histidine reacts approximately four times faster than the other (histidyl) residues. In sharp contrast with the phenomenon observed in connection with CO2 hydration and HCO3- dehydration, acrolein improves the catalytic efficiency of the enzyme toward p-nitrophenyl acetate hydrolysis and acetaldehyde hydration, with the relative activity increasing by approximately 12 and 34%, respectively. The widely differing effects imparted by the same reagent represent the first step toward differential control of the specificity of carbonic anhydrase II.

• Authors: Y Pocker, N Janjić
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 263 Issue 13 Pg. 6169-76 (May 05 1988) ISSN: 0021-9258 [Print] United States
• PMID: 3129421 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Aldehydes
• Bicarbonates
• Carbon Dioxide
• Histidine
• Acrolein
• Carbonic Anhydrases

Topics

• Acrolein (metabolism)
• Aldehydes (metabolism)
• Algorithms
• Animals
• Bicarbonates (metabolism)
• Carbon Dioxide (metabolism)
• Carbonic Anhydrases (metabolism)
• Cattle
• Erythrocytes (enzymology)
• Histidine (metabolism)
• Hydrogen-Ion Concentration
• Kinetics
• Time Factors