DIDS decreases CSF HCO3- and increases breathing in response to CO2 in awake rabbits.

Abstract

An inhibitor of the HCO3-/Cl- exchange carrier protein, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) or vehicle was infused in mock cerebrospinal fluid (CSF) via the cisterna magna in conscious rabbits at 10 mumol/l for 40 min at 10 microliter/min. Neither treatment had any effect over 2-5 h on the non-CO2-stimulated CSF ion values or blood gases. With CO2 stimulation such that arterial PCO2 (PaCO2) was increased 25 Torr over 3 h, DIDS treatment significantly decreased the stoichiometrically opposite changes in CSF [HCO3-] and [Cl-] that normally accompany hypercapnia and reflect ionic mechanisms of CSF pH regulation. Expressed as delta CSF [HCO3-]/delta PaCO2, DIDS treatment decreased the CSF ionic response by 35%. In a separate paired study design DIDS administration via the same protocol had no effect on resting ventilation but significantly increased the ventilation and tidal volume responses to a 28-Torr increase in PaCO2. Expressed as change in minute ventilation divided by delta PaCO2, DIDS treatment produced a 39.6% increase. The results support the concept of a DIDS-inhibitable anion exchange carrier being involved in CSF pH regulation in hypercapnia and suggest a DIDS-related effect on the ventilatory response to CO2.

• Authors: E E Nattie, J M Adams
• Journal: Journal of applied physiology (Bethesda, Md. : 1985) (J Appl Physiol (1985)) Vol. 64 Issue 1 Pg. 397-403 (Jan 1988) ISSN: 8750-7587 [Print] United States
• PMID: 3128529 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bicarbonates
• Stilbenes
• Carbon Dioxide
• 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
• 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid

Topics

• 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
• 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid (analogs & derivatives, pharmacology)
• Acidosis, Respiratory (cerebrospinal fluid)
• Animals
• Bicarbonates (cerebrospinal fluid)
• Carbon Dioxide (pharmacology)
• Rabbits
• Respiration (drug effects)
• Stilbenes (pharmacology)