Expression of diagnostic 31/32 kilodalton proteins of Schistosoma mansoni as fusions with bacteriophage MS2 polymerase.

Abstract

An expression plasmid pEx34b was used to synthesise parts of the 31/32 kDa Schistosoma mansoni antigens as fusions with the amino terminus of the phage MS2 polymerase. Purified MS2-schistosome fusion proteins reacted specifically in an enzyme-linked immunosorbent assay with sera from S. mansoni-infected patients. The observation that the majority of human sera tested recognised schistosome-specific epitopes, but not the MS2 polymerase fragment, suggests that the fusion proteins are useful for the immunodiagnosis of schistosomiasis and might be incorporated in a serological test system based on recombinant antigens.

• Authors: M Q Klinkert, A Ruppel, R Felleisen, G Link, E Beck
• Journal: Molecular and biochemical parasitology (Mol Biochem Parasitol) Vol. 27 Issue 2-3 Pg. 233-9 (Jan 15 1988) ISSN: 0166-6851 [Print] Netherlands
• PMID: 3125431 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Recombinant Fusion Proteins
• Recombinant Proteins

Topics

• Animals
• Antibodies, Helminth (immunology)
• Antigens, Helminth (biosynthesis, genetics, immunology)
• Coliphages (genetics)
• Escherichia coli (metabolism)
• Humans
• Plasmids
• Recombinant Fusion Proteins (biosynthesis, immunology)
• Recombinant Proteins (biosynthesis)
• Schistosoma mansoni (analysis, genetics, immunology)
• Schistosomiasis mansoni (immunology)