Abstract |
Stimulation with bacterial lipopolysaccharide (LPS) of splenic B-lymphocytes infected in vitro with Friend virus complex increased the number of cells with replicating murine leukemia virus (MuLV) [i.e., infectious centers (IC)] up to 100-fold. Concanavalin A (Con A) did not have such an effect. However, the addition of Con A to the LPS-stimulated cultures decreased the number of IC. The inhibitory concentration of Con A (2.5 microgram/ml) was eightfold less than that capable of neutralizing the in vitro infectivity of MuLV (20 microgram/ml). The effect of Con A was not mediated by T-cells; the inhibition of infection was comparable with use of whole spleen cell suspensions from normal BALB/c mice, with T-cell-depleted cell suspensions, or with spleen cells with congenitally athymic nude mice. However, specific removal of Con A from the surface of B-cells with alpha- methyl-D-mannopyranoside prior to the infection reversed the inhibitory effect entirely. It is suggested that the lectin interferes with MuLV on the membrane of B-cells.
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Authors | D L Bowen, D D Isaak, J Cerny |
Journal | Journal of the National Cancer Institute
(J Natl Cancer Inst)
Vol. 62
Issue 6
Pg. 1497-502
(Jun 1979)
ISSN: 0027-8874 [Print] United States |
PMID | 312351
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Lipopolysaccharides
- Concanavalin A
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Topics |
- Animals
- B-Lymphocytes
(immunology)
- Cell Membrane
(immunology)
- Cell Transformation, Neoplastic
- Concanavalin A
(pharmacology)
- Female
- Friend murine leukemia virus
- In Vitro Techniques
- Lipopolysaccharides
(immunology)
- Male
- Mice
- Mice, Inbred BALB C
- Spleen
(immunology)
- Virus Replication
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