Inhibition of in vitro Friend murine leukemia virus infection of lipopolysaccharide-activated B-cells with concanavalin A.

Abstract

Stimulation with bacterial lipopolysaccharide (LPS) of splenic B-lymphocytes infected in vitro with Friend virus complex increased the number of cells with replicating murine leukemia virus (MuLV) [i.e., infectious centers (IC)] up to 100-fold. Concanavalin A (Con A) did not have such an effect. However, the addition of Con A to the LPS-stimulated cultures decreased the number of IC. The inhibitory concentration of Con A (2.5 microgram/ml) was eightfold less than that capable of neutralizing the in vitro infectivity of MuLV (20 microgram/ml). The effect of Con A was not mediated by T-cells; the inhibition of infection was comparable with use of whole spleen cell suspensions from normal BALB/c mice, with T-cell-depleted cell suspensions, or with spleen cells with congenitally athymic nude mice. However, specific removal of Con A from the surface of B-cells with alpha-methyl-D-mannopyranoside prior to the infection reversed the inhibitory effect entirely. It is suggested that the lectin interferes with MuLV on the membrane of B-cells.

• Authors: D L Bowen, D D Isaak, J Cerny
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 62 Issue 6 Pg. 1497-502 (Jun 1979) ISSN: 0027-8874 [Print] United States
• PMID: 312351 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Lipopolysaccharides
• Concanavalin A

Topics

• Animals
• B-Lymphocytes (immunology)
• Cell Membrane (immunology)
• Cell Transformation, Neoplastic
• Concanavalin A (pharmacology)
• Female
• Friend murine leukemia virus
• In Vitro Techniques
• Lipopolysaccharides (immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Spleen (immunology)
• Virus Replication