Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line.

Abstract

Prepro-thyrotropin-releasing hormone (TRH) messenger RNA was detected in the rat medullary thyroid carcinoma cell line CA77. The RNA of 1.6 kilobases comigrated with that found in rat hypothalamus. Using three radioimmunoassays specific for pro-TRH-derived peptides, we demonstrated that CA77 cells synthesize high levels of immunoreactive TRH and all of the other pro-TRH-derived peptides identified in hypothalamic tissue. The relative levels of the pro-TRH-derived peptides also indicate that CA77 cells process the TRH precursor in a manner similar to hypothalamic tissue. CA77 cells provide a promising model system for further studies of prepro-TRH gene regulation and post-translational maturation.

• Authors: K A Sevarino, P Wu, I M Jackson, B A Roos, G Mandel, R H Goodman
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 263 Issue 2 Pg. 620-3 (Jan 15 1988) ISSN: 0021-9258 [Print] United States
• PMID: 3121619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Messenger
• Thyrotropin-Releasing Hormone

Topics

• Animals
• Cell Line
• Hypothalamus (metabolism)
• RNA, Messenger (analysis)
• Radioimmunoassay
• Rats
• Thyroid Neoplasms (metabolism)
• Thyrotropin-Releasing Hormone (biosynthesis, genetics)