MiRNA-93-5p promotes the biological progression of gastric cancer cells via Hippo signaling pathway.

Abstract

OBJECTIVE:

To clarify the influence of microRNA-93-5p (miRNA-93-5p) on biological behaviors of gastric cancer (GC) cells and its regulatory effect on Hippo pathway.

MATERIALS AND METHODS:

SGC-7901 and HGC-27 cells were used for establishing miRNA-93-5p overexpression and downregulation model through transfection of miRNA-93-5p mimics or inhibitor, respectively. Relative levels of genes in Hippo pathway were determined in GC cells transfected with miRNA-93-5p mimics or inhibitor by quantitative Real-time polymerase chain reaction (qRT-PCR). Regulatory effects of miRNA-93-5p on proliferative, migratory and invasive abilities of GC cells were evaluated by cell counting kit-8 (CCK-8), colony formation and transwell assay, respectively.

RESULTS:

MiRNA-93-5p was markedly upregulated by transfection of miRNA-93-5p mimics into SGC-7901 cells, which was downregulated by transfection of miRNA-93-5p inhibitor into HGC-27 cells. Overexpression of miRNA-93-5p accelerated GC cells to proliferate, migrate and invade. Meanwhile, miRNA-93-5p overexpression in GC cells upregulated downstream genes in Hippo pathway, including CDX2, FOXM1 and CTGF.

CONCLUSIONS:

MiRNA-93-5p enhances proliferative, migratory and invasive abilities of GC cells by activating Hippo pathway, which may serve as a diagnostic and therapeutic target for GC.

• Authors: H Meng, Y-Y Li, D Han, C-Y Zhang
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 23 Issue 11 Pg. 4763-4769 (Jun 2019) ISSN: 2284-0729 [Electronic] Italy
• PMID: 31210305 (Publication Type: Journal Article)

Chemical References

• MIRN93 microRNA, human
• MicroRNAs
• Protein Serine-Threonine Kinases

Topics

• Cell Movement
• Cell Proliferation
• Cell Survival
• Gene Expression Profiling
• Hippo Signaling Pathway
• Humans
• MicroRNAs (genetics, metabolism)
• Protein Serine-Threonine Kinases (genetics, metabolism)
• Signal Transduction (genetics)
• Stomach Neoplasms (genetics, metabolism, pathology)
• Tumor Cells, Cultured