Abstract |
A strain of Trichomonas vaginalis (IR-78), recently isolated from a patient afflicted with recurrent symptomatic trichomoniasis, showed resistance to metronidazole, tinidazole, and nimorazole in vitro as well as in vivo. In a serial dilution test using cysteine monohydrochloride- peptone-liver infusion- maltose medium, T. vaginalis IR-78 was only resistant under aerobic conditions. Under anaerobic conditions it was as susceptible as the normal reference strain. The minimal lethal concentrations of metronidazole, tinidazole, and nimorazole for IR-78 were 100, 50, and 50 mug/ml aerobically and 0.4, 0.4, and 0.2 mug/ml anaérobically, respectively. The efficacy of metronidazole, tinidazole, and nimorazole was assessed in vivo by oral administration to mice simultaneously infected with IR-78 both subcutaneously and intraperitoneally. The CD(50) (dose needed to cure 50% of infections) of each compound was significantly higher for the subcutaneous than for the intraperitoneal infection. In contrast, there was little difference in CD(50) for these infections in mice inoculated with a susceptible trichomonas strain. The CD(50)'s for all three compounds against intraperitoneal and subcutaneous infections with IR-78 were 2 to >70 times higher than for susceptible strain E. Both forms of infection with IR-78 could always be cured with therapeutically acceptable doses of tinidazole and nimorazole; subcutaneous infections could not be cured with tolerated doses of metronidazole.
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Authors | J G Meingassner, J Thurner |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 15
Issue 2
Pg. 254-7
(Feb 1979)
ISSN: 0066-4804 [Print] United States |
PMID | 311617
(Publication Type: Journal Article)
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Chemical References |
- Nitroimidazoles
- Metronidazole
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Topics |
- Animals
- Drug Resistance, Microbial
- Female
- Metronidazole
(pharmacology, therapeutic use)
- Mice
- Microbial Sensitivity Tests
- Nitroimidazoles
(pharmacology, therapeutic use)
- Trichomonas Infections
(drug therapy)
- Trichomonas vaginalis
(drug effects)
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