Abstract |
We speculate that exosomes derived from human umbilical cord mesenchymal stromal cells (HUC-MSCs) will accumulate within tumors and have the potential for both tumor location or drug delivery. Methods: To determine proof of concept, HUC-MSC exosomes were labeled with an MRI contrast agent, gadolinium, or a near infrared dye. Exosome accumulation within ectopic osteosarcoma tumor-bearing mice was determined by 14.1 T MRI or bioimaging over 24-48 h after injection. In vitro studies examine the accumulation and physiological effect of exosomes on human and mouse osteosarcoma cell lines by MTT assay, confocal microscopy, and flow cytometry. Results: Systemic HUC-MSC exosomes accumulated continuously in tumor over a 24-48 h post-injection period. In contrast, synthetic lipid nanoparticles accumulate in tumor only for the first 3 h post-injection. Conclusion: These results suggest that HUC-MSCs exosomes accumulate within human or mouse osteosarcoma cells in vitro and in vivo over a 24 to 48 h after infusion.
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Authors | Javier Abello, Tuyen Duong Thanh Nguyen, Ramesh Marasini, Santosh Aryal, Mark Louis Weiss |
Journal | Theranostics
(Theranostics)
Vol. 9
Issue 8
Pg. 2325-2345
( 2019)
ISSN: 1838-7640 [Electronic] Australia |
PMID | 31149047
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Cell Line, Tumor
- Cells, Cultured
- Exosomes
(chemistry, metabolism, transplantation)
- Gadolinium
(chemistry, pharmacokinetics)
- Humans
- Infrared Rays
- Magnetic Resonance Imaging
(methods)
- Mesenchymal Stem Cells
(metabolism)
- Mice
- Mice, Nude
- Nanoparticles
(chemistry, metabolism)
- Optical Imaging
(methods)
- Osteosarcoma
(diagnostic imaging)
- Tissue Distribution
- Umbilical Cord
(cytology)
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