HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Granulocyte-macrophage colony-stimulating factor enhances the production of eosinophil chemotactic lymphokine by egg-associated granulomas of Schistosoma japonicum-infected mice.

Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) produced by splenic lymphocytes obtained from Schistosoma japonicum-infected mice was partially purified by a combination of DEAE anion-exchange chromatography, concanavalin A-Sepharose affinity chromatography, and high-pressure liquid chromatography. When this partially purified GM-CSF was added to the culture of isolated intact granulomas, eosinophil chemotactic factor (ECF) lymphokine production by granulomas was significantly enhanced. The partially purified GM-CSF also enhanced ECF lymphokine production by granuloma T cells cocultured with syngeneic macrophages and specific antigen. The partially purified GM-CSF itself had neither ECF activity nor a synergistic effect with ECF lymphokine. When normal splenic macrophages were preincubated with the partially purified GM-CSF, they potentiated the ECF production by granuloma T cells under the presence of specific antigen. Augmentation of ECF lymphokine production by partially purified GM-CSF was further confirmed by using T-cell clones that were established from granuloma T cells. These results suggest that T-cell-derived GM-CSF primarily activate macrophages so that these activated macrophages can cooperate more effectively with T lymphocytes to produce ECF. Such potentiation of macrophage-T-cell interaction by GM-CSF may be important in the mechanisms of granuloma formation during an acute stage of schistosomiasis.
AuthorsM Owhashi, H Maruyama, Y Nawa
JournalInfection and immunity (Infect Immun) Vol. 55 Issue 9 Pg. 2042-6 (Sep 1987) ISSN: 0019-9567 [Print] United States
PMID3114144 (Publication Type: Journal Article)
Chemical References
  • Culture Media
  • Interleukin-3
  • Lymphokines
  • eosinophil stimulating promoter
Topics
  • Animals
  • Antigen-Presenting Cells (physiology)
  • Cells, Cultured
  • Clone Cells (physiology)
  • Culture Media
  • Eosinophils
  • Granuloma (physiopathology)
  • Interleukin-3 (pharmacology)
  • Lymphokines (biosynthesis)
  • Macrophages (physiology)
  • Mice
  • Schistosoma japonicum
  • Schistosomiasis japonica (physiopathology)
  • T-Lymphocytes (physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: