Knockdown of hsa_circ_0023028 inhibits cell proliferation, migration, and invasion in laryngeal cancer by sponging miR-194-5p.

Abstract

Emerging evidences have proposed that circular RNAs (circRNAs) play a major role in carcinogenesis. Hsa_circ_0023028 has been reported to be aberrantly expressed in laryngeal cancer (LCa). However, the role and the mechanism of hsa_circ_0023028 in LCa have not been adequately studied. In the present study, we demonstrated that hsa_circ_0023028 expression was up-regulated in LCa tissues and cell lines. miR-194-5p was down-regulated in LCa cells. Functionally, knockdown of hsa_circ_0023028 inhibited the proliferation, migration, and invasion of LCa cells, as evidenced by the reduced number of 5-Ethynyl-2'-deoxyuridine (EdU)-positive cells and decreased number of migrated and invaded cells. Additionally, hsa_circ_0023028 was identified as an miR-194-5p sink. A negative correlation between miR-194-5p and hsa_circ_0023028 expression was observed in LCa tissues. Besides, down-regulation of miR-194-5p attenuated the inhibitory effects of hsa_circ_0023028 silencing on LCa cell proliferation, migration, and invasion. In summary, hsa_circ_0023028 functions as an miR-194-5p sponge to promote the proliferation, migration, and invasion of LCa cells.

• Authors: Xiaofeng Chen, Xiaoqing Su, Chuansai Zhu, Jing Zhou
• Journal: Bioscience reports (Biosci Rep) Vol. 39 Issue 6 (06 28 2019) ISSN: 1573-4935 [Electronic] England
• PMID: 31123169 (Publication Type: Journal Article)
• Copyright: © 2019 The Author(s).

Chemical References

• MIRN194 microRNA, human
• MicroRNAs
• RNA, Circular
• RNA, Neoplasm

Topics

• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Humans
• Laryngeal Neoplasms (genetics, metabolism, pathology)
• MicroRNAs (biosynthesis, genetics)
• Neoplasm Invasiveness
• RNA, Circular (genetics, metabolism)
• RNA, Neoplasm (genetics, metabolism)