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Immunocytochemical localization of factor VIII-related antigen in tumors of the human central nervous system.

Abstract
Using both immunohisto- and immunocytochemical techniques with periodate-lysine-paraformaldehyde (PLP) fixation, we have studied the distribution of Factor VIII-related antigen (FVIIIR:Ag) in 12 cases of tumors of the human central nervous system (CNS) and one sample of non-tumor brain tissue. FVIIIR:Ag was found both extracellularly and intracellularly. It was localized in the vascular lumen, between endothelial cells, and in the endothelial cell basement membrane. In the endothelial cell cytoplasm, FVIIIR:Ag was found in the endoplasmic reticulum, perinuclear space, and in intracytoplasmic vacuoles and vesicles. Characteristic of malignant tumors (six out of seven) was a strongly-positive dilated endoplasmic reticulum. This may reflect increased FVIIIR:Ag synthesis in the endothelial cells of malignant tumors. Only one of five benign tumors showed such staining. Six of 12 tumors and the non-tumor brain showed perinuclear FVIIIR:Ag. Both ad- and abluminal vesicles in the tumor endothelial cells contained FVIIIR:Ag suggesting that endocytosis, transcellular transport, and/or endocytosis, as well as FVIIIR:Ag synthesis occurs. The non-tumor brain showed normal capillary structure and very little FVIIIR:Ag immunoreactivity. The relationship of these FVIIIR:Ag abnormalities to the hypercoagulable state seen in some malignant brain tumor patients remains to be clarified.
AuthorsM Miyagami, B H Smith, P E McKeever, B M Chronwall, M A Greenwood, P L Kornblith
JournalJournal of neuro-oncology (J Neurooncol) Vol. 4 Issue 3 Pg. 269-85 ( 1987) ISSN: 0167-594X [Print] United States
PMID3104549 (Publication Type: Journal Article)
Chemical References
  • Antigens
  • von Willebrand Factor
  • Factor VIII
Topics
  • Antigens
  • Brain Neoplasms (blood supply, immunology, ultrastructure)
  • Endothelium (immunology)
  • Factor VIII (immunology)
  • Histocytochemistry
  • Humans
  • Immunochemistry
  • Meningeal Neoplasms (blood supply, immunology, ultrastructure)
  • Microscopy, Electron
  • von Willebrand Factor

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