Enhanced inhibition of anticancer drugs by human recombinant gamma-interferon for human renal cell carcinoma in vitro.

Abstract

We studied the inhibitory effect of seven anticancer drugs and alpha-, recombinant beta-, and recombinant gamma-interferons on the in vitro growth of two established human renal cell carcinomas and 16 renal cell carcinomas obtained from patients using monolayer culture and the double-layer soft agar system. Recombinant gamma-interferon was the most effective of three types of interferon. Combined treatment with recombinant gamma-interferon and some anticancer drugs inhibited the cell growth in both cell lines more than treatment with each drug alone. Treatment with recombinant gamma-interferon and cisplatinum or 5-fluorouracil following a 24-hour incubation with the interferon was more effective than when interferon and the drug were given simultaneously. Treatment using doxorubicin, cisplatinum, or vinblastine with recombinant gamma-interferon synergistically inhibited colony formation in 11 of the 16 renal cell carcinomas that showed clonal growth. Our results suggest that treatment with anticancer drugs in combination with recombinant gamma-interferon is effective for renal cell carcinoma.

• Authors: K Gohji, S Maeda, T Sugiyama, J Ishigami, S Kamidono
• Journal: The Journal of urology (J Urol) Vol. 137 Issue 3 Pg. 539-43 (Mar 1987) ISSN: 0022-5347 [Print] United States
• PMID: 3102760 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Recombinant Proteins
• Interferon-gamma

Topics

• Antineoplastic Agents (pharmacology)
• Carcinoma, Renal Cell (pathology)
• Colony-Forming Units Assay
• Humans
• In Vitro Techniques
• Interferon-gamma (pharmacology)
• Kidney Neoplasms (pathology)
• Recombinant Proteins (pharmacology)
• Tumor Stem Cell Assay