Abstract |
We have prepared monoclonal antibodies (MoAbs) with the specific ability to bind metal chelates such as 111In benzyl EDTA. One, 10, 50 and 100 micrograms MoAb CHA255 Kb = 4 X 10E9 was complexed with 111In BLEDTA II, BLEDTA IV, and benzyl EDTA and injected i.v. in Balb/c mice with KHJJ tumor. The biological half-life by whole body counting was profoundly altered for all three compounds; from minutes to hours with 10 micrograms; to days with 100 micrograms. Tumor uptake increased 50 fold at 24 h with increasing MoAb but satisfactory tumor concentrations (3% per g) and tumor/blood ratios (1.8:1) were obtained with an amount equivalent to 7 mg for a human. Blood level and whole body activity were decreased 30-50% within 3 h or i.v. injection of a " flushing" dose of unlabeled indium benzyl EDTA, increasing tumor/blood ratios to 50:1.
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Authors | D A Goodwin, C F Meares, G F David, M McTigue, M J McCall, J M Frincke, M R Stone, R M Bartholomew, J P Leung |
Journal | International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
(Int J Rad Appl Instrum B)
Vol. 13
Issue 4
Pg. 383-91
( 1986)
ISSN: 0883-2897 [Print] England |
PMID | 3098706
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Radioisotopes
- Indium
- Edetic Acid
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Topics |
- Animals
- Antibodies, Monoclonal
- Edetic Acid
- Half-Life
- Indium
(metabolism)
- Kinetics
- Mice
- Mice, Inbred BALB C
- Neoplasms, Experimental
(diagnostic imaging)
- Radioisotopes
- Radionuclide Imaging
- Tissue Distribution
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