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Monoclonal antibodies, L-35 and L-36, define novel T cell activation antigens.

Abstract
Two novel T cell specific activation antigens were characterized and were defined by monoclonal antibodies developed against mitogen-stimulated human T cells. These antigens, designated as L-35 and L-36 were expressed on both the CD 4(Leu-3) and the CD 8(Leu-2) subsets of activated but not resting T cells. Moreover these antigens were not expressed on a number of T, B, and myeloid tumor cell lines. L-35 and L-36 were expressed on interleukin 2 (IL 2)-dependent cloned T cell lines, and were weakly expressed on the T cell tumor line, HSB-2. L-35 was expressed on granulocytes and a small subset of thymocytes. SDS-PAGE analysis of 125I-labeled lysates from phytohemagglutinin (PHA)-activated T cells demonstrated that L-35 existed as a complex of 32,000 and 97,000 dalton polypeptides under reducing and nonreducing conditions. Anti-L-36 immunoprecipitated a 90,000 dalton structure from PHA-activated cell lysates prepared with CHAPS detergent. When immunoprecipitates were analyzed from [35S]methionine labeled lysates, anti-L-35 precipitated only the 97,000 dalton component, suggesting that the 32,000 dalton subunit of L-35 complex was not synthesized by the activated cell population. Furthermore anti-L-35 did not immunoprecipitate a 32,000 dalton component from 125I-labeled lysates of anti-Leu-4 or Con A-activated cells, suggesting that the 32,000 dalton component of the L-35 complex may represent a subunit of PHA. The 32,000 dalton protein could not, however, be precipitated from cells incubated with PHA for less than 1 day. These results suggested that anti-L-35 recognizes a 97,000 dalton structure expressed on activated T cells, and that upon activation by PHA, becomes associated with a subunit of this mitogen.
AuthorsV C Maino, M E Janszen
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 137 Issue 10 Pg. 3093-9 (Nov 15 1986) ISSN: 0022-1767 [Print] United States
PMID3095423 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Membrane Proteins
Topics
  • Antibodies, Monoclonal (immunology)
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface (immunology)
  • B-Lymphocytes (immunology)
  • Cell Line
  • Flow Cytometry
  • Humans
  • Kinetics
  • Lymphocyte Activation
  • Membrane Proteins (immunology)
  • Molecular Weight
  • Monocytes (immunology)
  • T-Lymphocytes (classification, immunology)

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