Abstract |
Hybrid genes containing mRNA encoding sequences for herpes virus thymidine kinase (tk), chloramphenicol acetyltransferase (CAT), or Drosophila alcohol dehydrogenase (Adh), ligated to truncated Drosophila melanogaster heat-shock protein 70 (hsp 70) gene promoters or to synthetic sequences containing one or several copies of a previously defined heat-shock consensus sequence, were transfected into cultured Drosophila line S3 cells. Each construction was then assayed for gene expression at 25 degrees C and 37 degrees C, using a CAT enzyme assay, slot blot hybridization, or S1 nuclease protection analysis. In the Drosophila cell transient expression assay system, we found that deletions extending beyond position -97, or synthetic constructions containing a single heat shock consensus sequence, were not induced by high-temperature shock. In constructions containing deletions extending to position -186, -130, or -97, in the hsp 70 promoter, and in synthetic constructions containing tandemly spaced heat-shock consensus sequences mRNA transcription was greatly induced by high temperature.
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Authors | E M Berger, D Torrey, C Morganelli |
Journal | Somatic cell and molecular genetics
(Somat Cell Mol Genet)
Vol. 12
Issue 5
Pg. 433-40
(Sep 1986)
ISSN: 0740-7750 [Print] United States |
PMID | 3094168
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Heat-Shock Proteins
- RNA, Messenger
- DNA
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Topics |
- Animals
- Base Sequence
- Cells, Cultured
- Chromosome Mapping
- DNA
(genetics)
- Drosophila melanogaster
(genetics)
- Genes
- Genes, Synthetic
- Heat-Shock Proteins
(genetics)
- Nucleic Acid Hybridization
- Promoter Regions, Genetic
- RNA, Messenger
(genetics)
- Transfection
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