Effects and possible mechanism of Ruyiping formula application to breast cancer based on network prediction.

Abstract

Ruyiping (RYP), a Chinese herbal formula, can remove toxin and clear nodular, showing ability of preventing postoperative recurrence of breast cancer. In this study, network was performed to predict possible targets, genes and pathways associated with RYP and breast cancer. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were used to quantitatively study RYP formula and its single herbs. MTT methods, Luciferase reporter systems, zebrafish model and western blotting were respectively adopted to verify network prediction. Results showed that the quality of RYP could be controlled and icariin could be selected as mark ingredient; RYP expressed anti-breast tumor effects, which could be associated with inhibiting expression of Transforming Growth Factor β (TGFβ), promoting cells apoptosis and anti-angiogenesis. Parts of these results were consistent with network predictions in some degree, but not all. Network can help us narrow areas, focus on crucial factors, save money as well as time, but the results predicted by network should be confirmed by further experiments.

• Authors: Rui-Fang Xie, Sheng Liu, Ming Yang, Jia-Qi Xu, Zhi-Cheng Li, Xin Zhou
• Journal: Scientific reports (Sci Rep) Vol. 9 Issue 1 Pg. 5249 (03 27 2019) ISSN: 2045-2322 [Electronic] England
• PMID: 30918282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Transforming Growth Factor beta
• ruyiping

Topics

• Animals
• Apoptosis (drug effects, genetics)
• Breast Neoplasms (drug therapy)
• Chromatography, High Pressure Liquid
• Chromatography, Thin Layer
• Drugs, Chinese Herbal (chemistry, therapeutic use)
• Female
• Humans
• MCF-7 Cells
• Neoplasm Recurrence, Local (drug therapy)
• Transforming Growth Factor beta (metabolism)
• Zebrafish