Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro.

Abstract

BACKGROUND:

Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, <10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study.

METHODS:

Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied.

RESULTS:

Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level.

CONCLUSION:

In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

• Authors: Rizwan Wahab, Neha Kaushik, Farheen Khan, Nagendra Kumar Kaushik, Su-Jae Lee, Eun Ha Choi, Abdulaziz A Al-Khedhairy
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 14 Pg. 1131-1148 ( 2019) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 30863050 (Publication Type: Journal Article)

Chemical References

• beta Catenin
• Gold
• Adenosine Triphosphate

Topics

• Adenosine Triphosphate (metabolism)
• Carcinogenesis (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Cell Self Renewal
• Cell Survival
• Down-Regulation
• Glioma (pathology)
• Gold (chemistry)
• HEK293 Cells
• Humans
• Metal Nanoparticles (chemistry, ultrastructure)
• Neoplasm Invasiveness
• Neoplastic Stem Cells (metabolism, pathology)
• Particle Size
• Phenotype
• Quantum Dots (chemistry, ultrastructure)
• Signal Transduction
• Spheroids, Cellular (metabolism, pathology)
• X-Ray Diffraction
• beta Catenin (metabolism)