Ecdysteroid-regulated heat-shock gene expression during Drosophila melanogaster development.

Abstract

Peaks in hsp 26, 28, and 83 RNA levels are correlated with peaks in ecdysteroid titers during mid-embryogenesis, pupariation, and mid-pupation, and with a peak in the level of RNA from the 74EF ecdysone puff at pupariation. Inhibition of the ecdysteroid peak at pupariation by temperature shift of the conditionally ecdysteroid-deficient strain ecd-1 was followed by a disappearance of hsp 26 RNA and a decline in hsp 83 RNA level; subsequent addition of exogeneous 20-OH-ecdysone to the temperature-shifted strain resulted in a severalfold increase in hsp 83 RNA level, and a dramatic increase in that of hsp 26. These results are consistent with the induction of the hsp 83, 28, and 26 genes by ecdysteroid at several developmental stages.

• Authors: S R Thomas, J A Lengyel
• Journal: Developmental biology (Dev Biol) Vol. 115 Issue 2 Pg. 434-8 (Jun 1986) ISSN: 0012-1606 [Print] United States
• PMID: 3086161 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Heat-Shock Proteins
• Juvenile Hormones
• Ecdysterone

Topics

• Animals
• Drosophila melanogaster (drug effects, genetics, growth & development)
• Ecdysterone (pharmacology)
• Genes (drug effects)
• Heat-Shock Proteins (genetics, isolation & purification)
• Juvenile Hormones (physiology)
• Mutation