Abstract | BACKGROUND: METHODS: Cell proliferation and viability were detected by EdU and MTS assays. Cell cycle distribution was measured by flow cytometry. Migration and invasion were evaluated by scratch and transwell assays. Apoptotic levels were detected by TUNEL staining and western blotting. The protein levels of EGFR/RAS/RAF/ MEK/ERK signaling pathway were detected by western blotting. The in vivo results were determined by tumor xenografts in nude mice. The in vivo proliferation, tumor microvessel density, and apoptosis were detected by immunohistochemistry. RESULTS: CONCLUSIONS: EGCG could reduce the growth and increase the apoptosis of human thyroid carcinoma cells through suppressing the EGFR/RAS/RAF/ MEK/ERK signaling pathway. EGCG can be developed as an effective therapeutic agent for the treatment of thyroid cancer.
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Authors | Dongdong Wu, Zhengguo Liu, Jianmei Li, Qianqian Zhang, Peiyu Zhong, Tieshan Teng, Mingliang Chen, Zhongwen Xie, Ailing Ji, Yanzhang Li |
Journal | Cancer cell international
(Cancer Cell Int)
Vol. 19
Pg. 43
( 2019)
ISSN: 1475-2867 [Print] England |
PMID | 30858760
(Publication Type: Journal Article)
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