Abstract |
The present study was aimed to develop an effective nanoliposomal vaccine delivery system with P435 HER2/neu-derived peptide conjugated to Maleimide-PEG2000-DSPE. The nanoliposome formulation composed of DSPC/ DSPG/Chol/DOPE and monophosphoryl lipid A was used as an adjuvant. Liposomal formulations were prepared and their physical properties were characterized. Anti-tumoral efficacy of formulations was evaluated by immunization of tumor-bearing BALB/c mice and the generated immune response was studied by using ELISpot and flow cytometry analysis. The results of the study demonstrated Lip + DOPE + P535 formulation caused the lowest tumor size and the longest survival time in TUBO mice model and could make it a promising candidate in developing effective vaccines against HER2-positive breast cancers.
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Authors | Niloofar Farzad, Nastaran Barati, Amir Abbas Momtazi-Borojeni, Mona Yazdani, Atefeh Arab, Atefeh Razazan, Sheida Shariat, Mercedeh Mansourian, Azam Abbasi, Zahra Saberi, Ali Badiee, Seyed Amir Jalali, Mahmoud Reza Jaafari |
Journal | Artificial cells, nanomedicine, and biotechnology
(Artif Cells Nanomed Biotechnol)
Vol. 47
Issue 1
Pg. 665-673
(Dec 2019)
ISSN: 2169-141X [Electronic] England |
PMID | 30829072
(Publication Type: Journal Article)
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Chemical References |
- Cancer Vaccines
- Liposomes
- Peptides
- Erbb2 protein, mouse
- Receptor, ErbB-2
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Topics |
- Animals
- Cancer Vaccines
(chemistry, immunology, pharmacology)
- Cell Line, Tumor
- Female
- Liposomes
- Mammary Neoplasms, Experimental
(immunology, prevention & control)
- Mice, Inbred BALB C
- Nanoparticles
(chemistry, therapeutic use)
- Peptides
(chemistry, immunology, pharmacology)
- Receptor, ErbB-2
(chemistry, immunology, therapeutic use)
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